Human Factor IIa Alpha Thrombin Purified from Innovative Research has been prepared from human prothrombin, activated with Factor Xa, Va, and phospholipid, and purified to remove the activator enzymes. This is a frozen liquid buffered in 50 mM sodium citrate/ 0.2 M NaCl/ 0.1% PEG-8000/ pH 6.5, with a concentration of 3mg/ml. Complete activation was observed by SDS-PAGE analysis.
Thrombin (Coagulation Factor IIa) is created by the cleaving of Prothrombin (Coagulation Factor II) by Factor Xa/V during the clotting process. Thrombin acts as a catalyst in various coagulation reactions, including converting fibrinogen into fibrin, activating factors XI, VIII, V, and XIII, and promoting platelet activation/aggregation by activating protease-activated receptors. Thrombin is inactivated by antithrombin.
More Details:
- Source: Human Plasma
- Purification: Prepared from human plasma, activated with Factor Xa/Va and phospholipid, then purified to remove the activator enzymes.
- Purity: Complete activation observed by SDS-PAGE analysis. Minimum activity of 2700 NIH units/mg as compared to NIH standard Thrombin.
- Storage Conditions: -60 to -80C
At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
Please note – this product replaces our old product number IHaT (same product, new number).
